beta-Glucosidase, exo-beta-glucanase and pyridoxine transglucosylase activities of rice BGlu1

β-Glucosidase, exo-β-glucanase and pyridoxine transglucosylase activities of rice BGlu1

Rodjana OPASSIRI*, Yanling HUA†, Onnop WARA-ASWAPATI*, Takashi AKIYAMA‡, Jisnuson SVASTI§, Asim ESEN¶ and James R. KETUDAT CAIRNS*1 *Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand, †Center for Scientific and Technological Equipment, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand, ‡Department of Low Temperature Science, National...

Expression of cmg1, an exo-beta-1,3-glucanase gene from Coniothyrium minitans, increases during sclerotial parasitism.

During sclerotial infection of Sclerotinia sclerotiorum the mycoparasite Coniothyrium minitans penetrates through the host cell wall, which contains beta-1,3-glucan as its major component. A PCR-based strategy was used to clone a beta-1,3-glucanase-encoding gene, designated cmg1, from a cDNA library of the fungus. The nucleotide and deduced amino acid sequences of this gene showed high levels o...

ascospore thermoresistance . exo - 1 , 3 - beta - glucanase which contributes to encodes a sporulation - specific

Isolation and characterization of the K5-type yeast killer protein and its homology with an exo-beta-1,3-glucanase.

K5-type yeast killer protein in the culture supernatant of Pichia anomala NCYC 434 cells was concentrated by ultrafiltration and purified to homogeneity by ion-exchange chromatography with a POROS HQ/M column followed by gel filtration with a TSK G2000SW column. The protein migrated as a single band on discontinuous gradient SDS-PAGE and had a molecular mass of 49,000 Da. The pI value of the K5...

Discovery and characterization of a distinctive exo-1,3/1,4-{beta}-glucanase from the marine bacterium Pseudoalteromonas sp. strain BB1.

Marine bacteria residing on local red, green, and brown seaweeds were screened for exo-1,3-β-glucanase (ExoP) activity. Of the 90 bacterial species isolated from 32 seaweeds, only one, a Pseudoalteromonas sp., was found to display such activity. It was isolated from a Durvillaea sp., a brown kelp known to contain significant amounts of the storage polysaccharide laminarin (1,3-β-D-glucan with s...